报告人简介：

Dr. Wang is an associate professor in the Department of Internal Medicine and  the Michigan Nanotechnology Institute for Medicine and Biological Sciences. She  has morethan 20 years of research and teaching experience in autoimmune thyroid  diseaseand thyroid cancer. In the last ten years, she has actively extended  herresearch to include functionalized nanoparticles as a diagnostic  andtherapeutic approach for cancer cell targeting and imaging. Dr. Wang has  beenthe principal investigator of a project funded by the Department of  Defensethat utilizes nanoemulsion as a wound and burn decontamination agent. She  isalso as a co-investigator for the nanoemulsion adjuvant development  programfunded by BAA-NIAID.

报告内容简介：

Many breast and lung cancersoverexpress human epidermal growth factor  receptor-2 (HER-2). As such, in thisstudy we sought to enhance and specifically  design early detection imagingagents against these cancers by modulating the  humanized anti-HER-2 antibody Herceptin with gadolinium gold dendrimer entrapped  nanoparticles (Gd-AuDENP).Able to enhance computerizedtomography and magnetic  resonance contrast for detecting cancers early, Gd-AuDENPs were synthesized by  entrapping gold nanoparticles (AuNPs) in G5 PAMAMdendrimers using sodium  borohydride reduction chemistry while gadolinium waschelated into DOTA  conjugated to the dendrimer. Herceptin was modified with a5-azidopentanoic acid  linker and linked to the G5 PAMAM dendrimer via copperclick chemistry. Treatment  with Gd-Au DENP-Herceptin in vitro demonstrated the conjugate specifically and  exclusively binds to cell lines overexpressingHER-2. Subsequent internalization  occurred, whereas untargeted Gd-Au DENPsneither bound to nor were internalized  into cells lines overexpressing HER-2.These data therefore show a facile method  for conjugation of Herceptin to adendrimer based imaging agent with capacity for  versatile modification viaclick chemistry. This conjugate could serve in the  future simultaneously as adrug delivery agent and locator especially of  metastatic and early stagecancers.